Reverse Transcriptase

Key Advantage: Heat-Resistant, Lyophilization-Ready Reverse Transcriptase for Complex RNA Templates

• Feature
  Hifair™ V Reverse Transcriptase is an engineered upgrade of the Hieff™ M-MLV (H-) enzyme, optimized for enhanced thermal stability, high cDNA synthesis capacity (up to 10 kb), and glycerol-free formulation. It operates efficiently at elevated temperatures (up to 60°C), making it ideal for difficult RNA templates with strong secondary structures.

• Advantage
  With 70% residual activity maintained after 30 minutes at 58°C, Hifair™ V demonstrates superior thermostability compared to leading brands. This improves reverse transcription efficiency and fidelity, especially for complex or low-abundance RNA. Its broad input range (1 pg to 1 μg total RNA) supports highly sensitive RT-qPCR assays.

• Benefit
  Researchers can expect reliable performance across a wide dynamic range, improved detection sensitivity, and better amplification of GC-rich or structured RNA templates. The glycerol-free format also makes it an excellent choice for lyophilized formulations, facilitating room temperature storage and transport.