UltraScript® Reverse Transcriptase & cDNA Synthesis Kits

UltraScript® Reverse Transcriptase is a robust and thermostable modified MMLV reverse transcriptase engineered to enhance cDNA synthesis speed and yield with accurate transcript representation.

The enzyme allows highly sensitive reverse transcription of total, poly A⁺, and viral RNA sequences, making it an ideal component of RT-qPCR-based assays. Available as a stand-alone enzyme, or as a ready mix comprising reverse transcriptase, buffer system and, optionally, with an optimised blend of random hexamers with anchored oligo(dT) primers. All product formulations include a premixed potent RNase inhibitor to ensure template RNA integrity and minimise pipeting. This reverse transcriptase provides unbiased, fast, efficient and sensitive cDNA synthesis over a broad range of RNA template concentrations. 

Features

• Unbiased representation of 5’ and 3’ mRNA transcript ends
• Reduced RNase H activity 
• Thermostable reverse transcriptase 45 °C to 55 °C
• Advanced RNase inhibitor
• High cDNA yields from as little as 4 pg template RNA
• Accurate reverse transcription of GC-rich templates
• Sensitive detection of low copy number transcripts
• Advanced buffer chemistry including Mg and dNTPs
• Both premixed or separate oligo cDNA synthesis kits available